Analysis of 2-aminoacetophenone in wine using a stable isotope dilution assay and multidimensional gas chromatography–mass spectrometry
- 作者：Hans-Georg Schmarr ; Sebastian Ganß ; Wei Sang ; Theodoros Potouridis
- 关键词：Untypical aging off-flavor ; UTA ; 2-Aminoacetophenone ; MDGC– ; MS ; MCSS ; Stable isotope dilution analysis ; Wine
- 刊名：Journal of Chromatography A
- 出版年：2007
- 期刊代码：47_00219673
- 类别：ch
- 出版时间：25 May 2007
- 卷：1150
- 期：1-2
- 页码：78-84
- 文件大小：427 K
摘要
The key compound in the wine off-flavor phenomenon “Untypische Alterungsnote” (UTA) is 2-aminoacetophenone (AAP). A new method for its quantitative analysis in wine is described. The analysis is based on solid phase extraction with LiChrolut EN as sorbent material followed by multidimensional gas chromatography coupled to mass spectrometry (MDGC–MS). Matrix effects due to the complex wine aroma composition could be overcome by an alkaline washing step of the extract and the use of selected ion monitoring as detection mode. Quantification is based on a stable isotope dilution assay using deuterated 2-aminoacetophenone as internal standard.