Rational synthesis of 1,3-diolein by enzymatic esterification

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• 作者：Zhang-Qun Duan^a ; ^b ; ^{bioduan@gmail.com} ; Wei Du^b ; De-Hua Liu^b
• 关键词：Rational synthesis ; 1 ; 3-Diolein ; Esterification ; Response surface methodology
• 刊名：Journal of Biotechnology
• 出版年：2012
• 期刊代码：53_01681656
• 类别：imm
• 出版时间：31 May, 2012
• 卷：159
• 期：1-2
• 页码：44-49
• 文件大小：569 K

摘要

t-Butanol was an excellent reaction medium for enzyme-mediated esterification of oleic acid with glycerol for 1,3-diolein preparation which has been proved by our group. Herein, to achieve the rational synthesis of 1,3-diolien, response surface methodology was applied to examine the effects of the significant variables and their reciprocal effects on the product synthesis. Under the optimal conditions (62.4 掳C, 0.75 g Novozym 435, substrate molar ratio (oleic acid/glycerol) 2.4 and 4.8 g t-butanol), the diolein yield of 87.4%could be achieved, and the value of 1,3-diolien/diolein was as high as 87.8%, which were quite close to the predicted values. Compared with the results of our previous single factor experiment, although the values of diolein yield and 1,3-diolien/diolein could not be improved markedly, the enzyme dosage and the reaction medium were spared by 25%and 20%, respectively, which was a remarkable improvement of the enzymatic process.