Kinin and histamine stimulate Cl⁻ secretion in gerbil middle ear epithelium: connection to otitis media

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• 作者：Furukawa ; Masayuki ; Suzuki ; Hideaki ; Ikeda ; Katsuhisa ; Oshima ; Takeshi ; Yamaya ; Mutsuo ; Sasaki ; Hidetada ; et. al.
• 关键词：Short-circuit current ; Intracellular Ca²⁺ concentration ; Lysyl-bradykinin ; H₁ histamine receptor ; B₂ bradykinin receptor
• 刊名：Hearing Research
• 出版年：1999
• 期刊代码：111_03785955
• 类别：med
• 出版时间：June, 1999
• 卷：132
• 期：1-2
• 页码：109-116
• 文件大小：304 K

摘要

The effects of bradykinin (BK) and histamine on transepithelial ion transport in primary cultures of gerbil middle ear epithelium were investigated. Lysyl-bradykinin (lys-BK) elicited a transient increase in short-circuit current (I_sc) when added to apical or basolateral surfaces. Lys-BK had a larger effect than BK or des-arg⁹-BK on both epithelial surfaces. Histamine induced a transient increase in I_sc only when added to the basolateral surface. Mepyramine, an H₁ histamine antagonist, greatly reduced the histamine-induced I_sc. The H₂ and H₃ histamine antagonists were both ineffective for inhibiting the I_sc responses to histamine. Diphenylamine-2-carboxylate or 4,4′-diisothiocyanostilbene-2,2′-disulfonic acid, Cl⁻ channel blockers, significantly blocked the I_sc responses to lys-BK or histamine. The Ca²⁺-mobilizing action of lys-BK and histamine was also investigated in single middle ear epithelial cells. BK and histamine induced an increase in the intracellular Ca²⁺ concentration. 1,2-Bis-(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid-acetoxymethyl ester, a calcium chelator, greatly reduced the increase in the I_sc responses to lys-BK or histamine. These data indicate that BK and histamine activate intracellular Ca²⁺-dependent mechanisms, leading to apical Cl⁻ secretion in the cultured gerbil middle ear epithelium via B₂ BK receptors and H₁ histamine receptors.