Production of alkyl glucoside from cellooligosaccharides using yeast strains displaying Aspergillus aculeatus β-glucosidase 1
- 作者：Junji Ito ; Takumi Ebe ; Seiji Shibasaki ; Hideki Fukuda ; Akihiko Kondo
- 关键词：β ; -Glucosidase ; Alkyl glucoside ; Cell surface engineering ; Whole-cell biocatalyst
- 刊名：Journal of Molecular Catalysis B ; Enzymatic
- 出版年：2007
- 期刊代码：55_13811177
- 类别：ce
- 出版时间：16 November 2007
- 卷：49
- 期：1-4
- 页码：92-97
- 文件大小：322 K
摘要
For efficient alkyl glucoside production from cellooligosaccharides, we constructed a yeast strain for alkyl glucoside synthesis by genetically inducing the display of β-glucosidase 1 (BGL1) from the filamentous fungus Aspergillus aculeatus No. F-50 on the cell surface. The localization of BGL1 on the cell surface was confirmed by immunofluorescence microscopy. The yeast strain displaying BGL1 catalyzed alkyl glucoside synthesis from p-nitrophenyl β-d-glucoside and primary alcohols. The highest yield of alkyl glucoside was 27.3%of the total sugar. The substrate specificities of the BGL1-displaying yeast strain and almond β-glucosidase were compared using different-chain-length cellooligosaccharides. The BGL1-displaying yeast showed efficient alkyl glucoside production from not only glucose but also cellohexaose. This yeast is applicable as a whole-cell biocatalyst for alkyl glucoside production from cellulose hydrolysates.