Repeated fermentation from raw starch using Saccharomyces cerevisiae displaying both glucoamylase and 伪-amylase

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• 作者：Syun-ichi Yamakawa^a ; Ryosuke Yamada^b ; Tsutomu Tanaka^b ; Chiaki Ogino^a ; Akihiko Kondo^a ; ^{akondo@kobe-u.ac.jp}
• 关键词：Ethanol ; Yeast ; Amylase ; Starch ; Repeated fermentation
• 刊名：Enzyme and Microbial Technology
• 出版年：2012
• 期刊代码：26_01410229
• 类别：ce
• 出版时间：10 May, 2012
• 卷：50
• 期：6-7
• 页码：343-347
• 文件大小：387 K

摘要

A diploid yeast strain displaying both 伪-amylase and glucoamylase was developed for repeated fermentation from raw starch. First, the construct of 伪-amylase was optimized for cell surface display, as there have been no reports of 伪-amylase-displaying yeast. The modified yeast displaying both glucoamylase and 伪-amylase produced 46.5 g/l of ethanol from 200 g/l of raw corn starch after 120 h of fermentation, and this was 1.5-fold higher when compared to native 伪-amylase-displaying yeast. Using the glucoamylase and modified 伪-amylase co-displaying diploid strain, we repeated fermentation from 100 g/l of raw starch for 23 cycles without the loss of 伪-amylase or glucoamylase activity. The average ethanol productivity and yield during repeated fermentation were 1.61 g/l/h and 76.6%of the theoretical yield, respectively. This novel yeast may be useful for reducing the cost of bio-ethanol production and may be suitable for industrial-scale bio-ethanol production.