A disposable voltammetric immunosensor based on magnetic beads for early diagnosis of soybean rust

详细信息	查看全文 | 推荐本文 |

• 作者：R.K. Mendes^a ; ^{renatavalente@puc-campinas.edu.br}  ; [Author Vitae] ; S. Laschi^b  ; [Author Vitae] ; D.R. Stach-Machado^c  ; [Author Vitae] ; L.T. Kubota^d  ; [Author Vitae] ; G. Marrazza^b  ; [Author Vitae]
• 关键词：Immunosensor ; Magnetic beads ; Screen-printed electrode ; Soybean rust
• 刊名：Sensors and Actuators B ; Chemical
• 出版年：2012
• 期刊代码：161_09254005
• 类别：et
• 出版时间：20 May, 2012
• 卷：166-167
• 期：Complete
• 页码：135-140
• 文件大小：572 K

摘要

Asian soybean rust is a fungal disease caused by Phakopsora pachyrhizi which is a virulent pathogen that can cause losses up to 80%in culture, reducing value and product quality. Early identification of fungus in the plant is the better way to prevent economic losses as well as spreading to neighboring farming, avoiding a rapid epidemic. In this paper, a disposable electrochemical immunosensor was developed to detect the presence of fungus mycelium, ensuring early identification of disease stages, allowing swift action to be taken. The antibody anti-mycelium of P. pachyrhizi was immobilized on magnetic microbeads, modified with a protein G, via Fc portion of antibody. The immunotest was the sandwich type, using a secondary antibody labeled with phosphatase alkaline enzyme. The optimization of experimental conditions and the analytical parameters were evaluated. The developed disposable immunosensor presented a detection limit of 90 ng mL^鈭?. These studies provide new perspectives on using screen-printed carbon electrodes based on magnetic beads, for early diagnosis of soybean rust for analysis in field.