Antitumor activities of quercetin and quercetin-5鈥?8-disulfonate in human colon and breast cancer cell lines

详细信息	查看全文 | 推荐本文 |

• 作者：Haisheng Zhang^a ; Min Zhang^a ; Linhong Yu^a ; Yan Zhao^b ; Nianwu He^a ; Xingbin Yang^a ; ^{xbyang@snnu.edu.cn}
• 关键词：Quercetin ; Quercetin-5&prime ; 8-disulfonate ; Antiproliferation ; Cell cycle ; Apoptosis ; ROS
• 刊名：Food and Chemical Toxicology
• 出版年：2012
• 期刊代码：20_02786915
• 类别：pha
• 出版时间：May, 2012
• 卷：50
• 期：5
• 页码：1589-1599
• 文件大小：1333 K

摘要

This study is designed to compare the anticancer effects of quercetin and its water-soluble sulfated derivative, quercetin-5鈥?8-disulfonate (QS), in human colon cancer LoVo cells and breast cancer MCF-7 cells. It was found that both quercetin and QS can inhibit the growth of cancer cells in a dose-dependent manner, with the IC₅₀ values of 40.2 and 28.0 渭M for LoVo cells and 30.8 and 19.9 渭M for MCF-7 cells, respectively, suggesting QS was more effective against the cancer cells than quercetin. Moreover, flow cytometric assay revealed that quercetin and QS could mediate the cell-cycle arrest principally in the S phase after 24 h of treatment with the two tumor cells. It was also found that 69.6%of LoVo cells and 90.6%of MCF-7 cells entered the early phase of apoptosis when treated with 100 渭M QS for 48 h. Furthermore, we firstly found the generation of ROS is a critical mediator in QS-induced cell growth inhibition. Taken together, the novel sulfated derivative of quercetin possesses strong antitumor activity via a ROS-dependent apoptosis pathway, and has the excellent potential to be developed into an antitumor precursor compound.