The DMFC test results under steady state conditions indicate that the novel nanostructure MEA exhibits higher performance compared to the conventional MEA in term of maximum power density and resistivity against methanol crossover from the anode side to the cathode side. Maximum power density of 105 mW cm鈭? was obtained by new PANI modified anode compared to 75 mW cm鈭? by conventional anode in the same conditions. The enhanced performance could be attributed to the higher activity of the PANI modified anode and lower methanol crossover caused by the PANI as barrier in the modified anode.