Acid DNases and their interest among apoptotic endonucleases

详细信息	查看全文 | 推荐本文 |

• 作者：Marie-France Counis ; Alicia Torriglia
• 关键词：Apoptosis ; DNases ; CAD ; pH mainly due to NA⁺/H⁺ exchanger ; Acidic DNases ; DNase II α ; DNase II β ; L-DNase II
• 刊名：Biochimie
• 出版年：2006
• 期刊代码：24_03009084
• 类别：bio
• 出版时间：December 2006
• 卷：88
• 期：12
• 页码：1851-1858
• 文件大小：276 K

摘要

Apoptosis is characterized by cell shrinkage, nuclear condensation and internucleosomal DNA cleavage. Besides the central role of caspases and other proteases, cell death triggers DNA degradation so that DNases have an active role in apoptotic cell death. The best-characterized apoptotic DNase is CAD, a neutral Mg-dependent endonuclease. Its activity is regulated by its inhibitor, ICAD, which is cleaved by caspases. Other neutral DNases have been shown to cleave nuclear DNA in apoptotic conditions: endonuclease G, GADD.

In cells, the cytosolic pH is maintained to 7.2, mostly due to the activity of the Na⁺/H⁺ exchanger. In many apoptotic conditions, a decrease of the intracellular pH has been shown. This decrease may activate different acid DNases, mostly when pH decreases below 6.5.

Three acidic DNases II are so far known: DNase II α, DNase II β and L-DNase II, a DNase II, derived from the serpin LEI (Leukocyte Elastase Inhibitor). Their activation during cell death is discussed in this review.