In vitro leishmanicidal activity of N-dodecyl-1,2-ethanediamine

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• 作者：Alexandre L. Neves Silva^a ; Camila M. Adade^b ; Fernanda M. Shoyama^a ; Cecí ; lio Purcino S. Neto^a ; Thaí ; s S. Padró ; n^b ; Mauro V. de Almeida^c ; Carlos A. de Magalhã ; es Rezende^c ; Claudio V. Silva^a ; Maria A. Souza^a ; ^{masouza@ufu.br}
• 关键词：Leishmania ; Leishmanicidal activity ; Diamine
• 刊名：Biomedicine and Pharmacotherapy
• 出版年：2012
• 期刊代码：36_07533322
• 类别：med
• 出版时间：April, 2012
• 卷：66
• 期：3
• 页码：180-186
• 文件大小：1536 K

摘要

Polyamine biosynthesis and inhibition in parasites have been an attractive chemotherapeutic approach in the design of novel antiparasitic drugs. We study in this work the effect of N-dodecyl-1,2-ethylenediamine (NDDE) on the morphology and replication of Leishmania using macrophages cultured from the peritoneal exudate of mice infected in vitro with three species of Leishmania: Leishmania聽(Leishmania)聽amazonensis, Leishmania聽(Viannia)聽brasiliensis and Leishmania聽(Leishmania)聽chagasi. The results showed that NDDE inhibited Leishmania amastigotes multiplication into inflammatory peritoneal cells in concentrations which were not toxic to mammalian cells (0.5-1 渭g/mL). An intracellular disorganization of the promastigote forms was observed by transmission electron microscopy after 3 to 24 h of treatment with 1 渭g/mL NDDE, suggesting that this compound affects the viability of the parasite by an autophagy pathway.