Donepezil attenuates excitotoxic damage induced by membrane depolarization of cortical neurons exposed to veratridine

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• 作者：Shigeru Akasofu ; Kohei Sawada ; Takashi Kosasa ; Hiroe Hihara ; Hiroo Ogura ; Akinori Akaike
• 关键词：Donepezil ; Veratridine ; NMDA receptor ; Na⁺ channel ; Intracellular sodium concentration ([Na⁺]_i) ; Intracellular calcium concentration ([Ca²⁺]_i) ; Excitatory amino acid ; Rat primary-cultured cortical neu
• 刊名：European Journal of Pharmacology
• 出版年：2008
• 期刊代码：24_00142999
• 类别：neu
• 出版时间：7 July 2008
• 卷：588
• 期：2-3
• 页码：189-197
• 文件大小：1264 K

摘要

Long-lasting membrane depolarization in cerebral ischemia causes neurotoxicity via increases of intracellular sodium concentration ([Na⁺]_i) and calcium concentration ([Ca²⁺]_i). Donepezil has been shown to exert neuroprotective effects in an oxygen–glucose deprivation model. In the present study, we examined the effect of donepezil on depolarization-induced neuronal cell injury resulting from prolonged opening of Na⁺ channels with veratridine in rat primary-cultured cortical neurons. Veratridine (10 µM)-induced neuronal cell damage was completely prevented by 0.1 µM tetrodotoxin. Pretreatment with donepezil (0.1–10 µM) for 1 day significantly decreased cell death in a concentration-dependent manner, and a potent NMDA receptor antagonist, dizocilpine (MK801), showed a neuroprotective effect at the concentration of 10 µM. The neuroprotective effect of donepezil was not affected by nicotinic or muscarinic acetylcholine receptor antagonists. We further characterized the neuroprotective properties of donepezil by measuring the effect on [Na⁺]_i and [Ca²⁺]_i in cells stimulated with veratridine. At 0.1–10 µM, donepezil significantly and concentration-dependently reduced the veratridine-induced increase of [Ca²⁺]_i, whereas MK801 had no effect. At 10 µM, donepezil significantly decreased the veratridine-induced increase of [Na⁺]_i. We also measured the effect on veratridine-induced release of the excitatory amino acids, glutamate and glycine. While donepezil decreased the release of glutamate and glycine, MK801 did not. In conclusion, our results indicate that donepezil has neuroprotective activity against depolarization-induced toxicity in rat cortical neurons via inhibition of the rapid influx of sodium and calcium ions, and via decrease of glutamate and glycine release, and also that this depolarization-induced toxicity is mediated by glutamate receptor activation.