Casein kinase 1 delta (CK1δ) interacts with the SNARE associated protein snapin
- 作者：Sonja Wolff ; Martin Stö ; ter ; Georgios Giamas ; Matthias Piesche ; Doris Henne-Bruns ; George Banting ; Uwe Knippschild
- 关键词：CK1δ ; Snapin ; Soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor ; Vesicle transport ; Phosphorylation ; Yeast two-hybrid system
- 刊名：FEBS Letters
- 出版年：2006
- 期刊代码：70_00145793
- 类别：bio
- 出版时间：27 November 2006
- 卷：580
- 期：27
- 页码：6477-6484
- 文件大小：1211 K
摘要
In this study we identified snapin as an interaction partner of the CK1 isoform delta (CK1δ) in the yeast two-hybrid system and localized the interacting domains of both proteins. The interaction of CK1δ with snapin was confirmed by co-immunoprecipitation. Snapin was phosphorylated by CK1δ in vitro. Both proteins localized in close proximity in the perinuclear region, wherein snapin was found to associate with membranes of the Golgi apparatus. The identification of snapin as a new substrate of CK1δ points towards a possible function for CK1δ in modulating snapin specific functions.