Enzymatic oxidation of the dopaminergic neurotoxin, 1 (R), 2(N)-Dimethyl-6,7-Dihydroxy-l,2,3,4-Tetrahydroisoquinoline, into 1,2(N)-Dimethyl-6,7-Dihydroxyisoquinolinium ion

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• 作者：Naoi ; Makoto ; Maruyama ; Wakako ; Jian ; Hua Zhang ; Takahashi ; Tsutomu ; Yulin ; Deng ; Dostert ; Philippe
• 关键词：N-methylsalsolinol ; semicarbazide-sensitive oxidase ; 1 ; 2-dimethyl-6 ; 7-dihydroxy-isoquinolinium
• 刊名：Life Sciences
• 出版年：1995
• 期刊代码：62_00243205
• 类别：imm
• 出版时间：August 4, 1995
• 卷：57
• 期：11
• 页码：1061-1066
• 文件大小：336 K

摘要

The dopamine-derived alkaloid, 1(R),2(N)-dimethyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline [N-methyl(R)salsolinol] was found to be oxidized enzymatically into the more cytotoxic species, 1,2(N)-dimethyl-6,7-dihydroxyisoquinolinium ion, using enzyme sample prepared from human brain cortex. The values of the Michaelis constant and of the maximum velocity were 912 μM and 1368 pmol/min/mg protein, respectively. The enzyme was not inhibited by inhibitors of monoamine oxidase, but was sensitive to semicarbazide. The oxidation is discussed in relation to the dopaminergic neurotoxicity of N-methyl-(R)salsolinol.