Comparison in antioxidant action between 伪-chitosan and 尾-chitosan at a wide range of molecular weight and chitosan concentration

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• 作者：Jooyeoun Jung ; Yanyun Zhao ; ^{yanyun.zhao@oregonstate.edu}
• 关键词：FC ; form of chitosan ; Mw ; molecular weight ; CS ; concentration of chitosan ; C&lowast ; coil-overlap concentration ; DDA ; degree of deacetylation ; DPPH ; 1 ; 1-diphenyl-2-picrylhydrazyl ; EDTA ; ethylenediaminetetraacetic acid ; T (%) ; transmittance ; [畏] ; intrins
• 刊名：Bioorganic and Medicinal Chemistry
• 出版年：2012
• 期刊代码：9_09680896
• 类别：ch
• 出版时间：1 May, 2012
• 卷：20
• 期：9
• 页码：2905-2911
• 文件大小：254 K

摘要

Antioxidant activity in 伪- and 尾-chitosan at a wide range of molecular weight (Mw) and chitosan concentration (CS) was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, reducing ability, chelating ability, and hydroxyl radical scavenging activity. The form of chitosan (FC) had significant (P <0.05) effect on all measurements except DPPH radical scavenging activity, and antioxidant activity was dependent on Mw and CS. High Mw (280-300 kDa) of 尾-chitosan had extremely lower half maximal effective concentrations (EC₅₀) than 伪-chitosan in DPPH radical scavenging activity and reducing ability. The 22-30 kDa of 伪- and 尾-chitosan showed significantly (P <0.05) higher activities in DPPH radical scavenging, reducing ability, and hydroxyl radical scavenging than samples at other Mw, while chelating ability was the highest in 4-5 kDa chitosan. CS had significant effect on all measurements and the effect was related to Mw. The antioxidant activity of 280-300 kDa chitosan was affected by coil-overlap concentrations (C^{鈭?/sup>) in the CS range of 4-10 mg/mL, forming entanglements. Reducing ability and hydroxyl radical scavenging activity were more predominant action in antioxidant activity of chitosan as shown by the lower EC₅₀ values than those in other antioxidant measurements.}