Use of gated ¹³N-NH₃ micro-PET to examine left ventricular function in rats

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• 作者：Mariusz K. Szymanski^a ; Silvana Kruizinga^a ; ^b ; Ren&eacute ; A. Tio^a ; Antoon T.M. Willemsen^b ; Michael A. Schä ; fers^d ; ^e ; Lars Stegger^e ; Rudi A. Dierckx^b ; Hans L. Hillege^a ; ^c ; Riemer H.J.A. Slart^b ; ^{r.h.j.a.slart@umcg.nl}
• 关键词：13N-NH3 micro-PET ; Cardiac function
• 刊名：Nuclear Medicine and Biology
• 出版年：2012
• 期刊代码：43_09698051
• 类别：ph
• 出版时间：July, 2012
• 卷：39
• 期：5
• 页码：724-729
• 文件大小：496 K

摘要

<h4 class="h4">Introductionh4>Myocardial perfusion gating techniques offer the possibility of measurement of left ventricular end-systolic (ESV) and end-diastolic volume (EDV) and left ventricular ejection fraction (LVEF) in clinical and preclinical trials. The aim of this study was to evaluate left ventricular volumes (LVV) and LVEF with ¹³N-NH₃ in comparison with the reference ¹⁸F-FDG in different rat models.<h4 class="h4">Methodsh4>

In this study, 18 male Wistar rats, 12 control rats and 6 rats with myocardial infarction (MI) were imaged with micro-PET. The ratswere scanned with gated ¹³N-NH₃ and ¹⁸F-FDG sequentially for the assessment of LVV and LVEF. A validated three-dimensional segmentation algorithm was used to calculate LVV and LVEF.<h4 class="h4">Resultsh4>

Mean LVEF measured with ¹³N-NH₃ was 45.6卤8.9 and 75.3卤9.4%, mean ESV was 0.40卤0.12 and 0.14卤0.11 ml, and mean EDVwas 0.53卤16 and 0.75卤0.18 ml for MI and control rats, respectively. Moderate to good correlations were observed between values of ¹³N-NH₃ and ¹⁸F-FDG for calculation of ESV [r=0.80, P<.0001, standard error of estimate (SEE)=0.10], EDV (r=0.63, P=.005, SEE=0.14) and LVEF (r=0.84, P<.0001, SEE=9.5). LVEF measured with ¹³N-NH₃ was significantly lower in MI rats in comparison to measurement with ¹⁸F-FDG (45.6卤8.9 vs 54.9卤9.3%; P=.04).<h4 class="h4">Conclusionh4>

Correlations were moderate to good for the assessment of ESV, EDV and LVEF between gated ¹³N-NH₃ and ¹⁸F-FDG. LVEF was underestimated with gated ¹³N-NH₃ in rats with myocardial infarction. In healthy rats, LV volumes and LVEF can be measured reproducibly with either approach.