Neuroprotective effects of adipose-derived stem cells against ischemic neuronal damage in the rabbit spinal cord

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• 作者：Jin Young Chung^a ; ¹ ; Woosuk Kim^b ; ¹ ; Wooseok Im^a ; Dae Young Yoo^b ; Jung Hoon Choi^c ; In Koo Hwang^b ; Moo-Ho Won^d ; In Bok Chang^e ; Byung Moon Cho^f ; Hyung Sik Hwang^g ; Seung Myung Moon^g ; ^{nsmsm@chol.com}
• 关键词：Transient spinal ischemia ; Adipose-derived stem cells ; Neuroprotection ; Brain-derived neurotrophic factor
• 刊名：Journal of the Neurological Sciences
• 出版年：2012
• 期刊代码：178_0022510x
• 类别：med
• 出版时间：15 June, 2012
• 卷：317
• 期：1-2
• 页码：40-46
• 文件大小：1798 K

摘要

Transplantation of adipose-derived stem cells (ASCs) is one of the possible therapeutic tools for ischemic damage. In this study, we observed the effects of ASCs against ischemic damage in the ventral horn of L_5-6 levels in the rabbit spinal cord. ASCs were isolated from rabbits, and cell type was confirmed by flow cytometry analysis, labeling with CM-DiI dye and differentiation into adipocytes in adipogenesis differentiation medium. ASCs were administered intrathecally into recipient rabbits (2 脳 10⁵) immediately after reperfusion following a 15-min aortic artery occlusion in the subrenal region. Transplantation of ASCs significantly improved functions of the hindlimb and morphology of the ventral horn of spinal cord although CM-DiI-labeled ASCs were not observed in the spinal cord parenchyma. In addition, transplantation of ASCs significantly increased brain-derived neurotrophic factor (BDNF) levels at 72 h after ischemia/reperfusion. These results suggest that transplantation of ASCs prevents motor neurons from spinal ischemic damage and reactive gliosis by increasing neurotrophic factors such as BDNF in the spinal cord.