Generation of transgenic zebrafish with liver-specific expression of EGFP-Lc3: A new in vivo model for investigation of liver autophagy

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• 作者：Jianzhou Cui^a ; ¹ ; Tina Huey-Fen Sim^b ; ¹ ; Zhiyuan Gong^b ; ^{dbsgzy@nus.edu.sg} ; Han-Ming Shen^a ; ^c ; ^{han-ming_shen@nuhs.edu.sg}
• 关键词：Zebrafish ; Autophagy ; Liver ; Lc3 ; Torin1 ; mTOR
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2012
• 期刊代码：159_0006291x
• 类别：bio
• 出版时间：1 June, 2012
• 卷：422
• 期：2
• 页码：268-273
• 文件大小：899 K

摘要

Transgenic expression of GFP-Lc3 is a useful tool for an in vivo model to monitor the formation of autophagosomes during the autophagy process. So far, two transgenic animals (mice and zebrafish) with expression of GFP-Lc3 have been reported. Liver is one of the most important organs for autophagy research. Here, we generated a transgenic zebrafish line with liver-specific EGFP-Lc3 expression. By exposing transgenic larvae to the autophagy inducer, Torin1, we observed a substantial increase in the number of EGFP-Lc3 puncta in the liver as well as the increase of Lc3-II protein. Notably, addition of a chloroquine (CQ) led to further increase of EGFP-Lc3 puncta in liver cells due to the blockage of lysosomal function and degradation stage of autophagy. Thus, the newly established transgenic line will be a useful in vivo model to investigate liver autophagy, and, in particular, the involvement of autophagy in basic biology and diseases in the liver.