- 作者：Kiyoshi Migita ; Yumi Maeda ; Seigo Abiru ; Minoru Nakamura ; Atsumasa Komori ; Seiji Miyazoe ; Kazuhiko Nakao ; Hiroshi Yatsuhashi ; Katsumi Eguchi ; Hiromi Ishibashi
- 刊名：Journal of Hepatology
- 出版年：2007
- 期刊代码：162_01688278
- 类别：med
- 出版时间：March 2007
- 卷：46
- 期：3
- 页码：381-386
- 文件大小：113 K
Background/Aims
Hepatitis B virus (HBV) induces liver cirrhosis (LC) and hepatocellular carcinoma (HCC) mainly by causing chronic necro-inflammatory hepatic disease. Our aim was to investigate the relationships between the polymorphisms of the interleukin-1B (IL-1B) promoter region and the interleukin-1 receptor antagonist gene (IL-1RN) and disease progression in an HBV-infected Japanese population.Methods
Genomic DNA was extracted from the peripheral blood of 237 HBV carriers. Polymorphisms in IL-1B and IL-1RN were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and PCR with confronting two-pair primers (PCR-CTPP) methods. These polymorphic sites include the promoter regions of IL-1B at positions −511 and −31, and IL-1RN variable tandem repeats.
Results
The IL-1B −31 and −511 loci were in complete linkage disequilibrium, and the frequency of the IL-1B −31 T carrier (IL-1B −31 T/T or T/C) was significantly higher in HBV carriers with LC compared to those without LC (LC; 86.1%vs non-LC; 72.1%, P = 0.009). There was no difference in the genotype distribution of the IL-1RN polymorphism.
Conclusions
This is the first report describing the association between IL-1B polymorphism and HBV-related hepatic fibrosis, and our data suggest that IL-1B polymorphisms may be related to disease progression of HBV-related hepatitis in Japan.