The anti-inflammatory activities of an extract and compounds isolated from Platycladus orientalis (Linnaeus) Franco in vitro and ex vivo

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• 作者：Si-Yang Fan^a ; ^b ; Hua-Wu Zeng^a ; Yue-Hu Pei^b ; Li Li^a ; ^b ; Ji Ye^a ; Yue-Xin Pan^a ; Ji-Gang Zhang^a ; Xing Yuan^a ; Wei-Dong Zhang^a ; ^c ; ^{Wdzhangy@hotmail.com}
• 关键词：5-HETE ; 5-hydroxy-eicosa-tetra-enoic acid ; LTB4 ; leukotriene B4 ; 12-HHT ; 12-hydroxyheptadeca-5 ; 8 ; 10-trienoic acid ; PGB2 ; prostaglandin B2 ; PGF2伪 ; prostaglandin F2伪 ; 5-LOX ; 5-lipoxygenase ; COX-2 ; cyclooxygenase-2 ; TNF-伪 ; tumor necrosis factor-alpha ; L
• 刊名：Journal of Ethnopharmacology
• 出版年：2012
• 期刊代码：26_03788741
• 类别：pha
• 出版时间：1 June, 2012
• 卷：141
• 期：2
• 页码：647-652
• 文件大小：847 K

摘要

Ethnopharmacological relevance

As a Chinese traditional herbal medicine, leaves of Platycladus orientalis (Linnaeus) Franco (LPO) are used to treat coughs, excessive mucus secretion, chronic bronchitis, bronchiectasis, and asthma, etc. The experiments were carried out to investigate their anti-inflammatory properties and mechanisms, which could support the Chinese traditional uses of treating inflammatory airway diseases.

Materials and methods

The anti-inflammatory activities of the chloroform fraction (CHL) and pure compounds of LPO were evaluated for their abilities to inhibit pro-inflammatory enzymes in vitro, and production of tumor necrosis factor-伪 (TNF-伪) and nitric oxide in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Furthermore, the arachidonic acid metabolites, stimulated by calcium ionophore A23187, were also determined by HPLC.

Results

For the first time, the assays of eicosanoids in intact cells showed that the CHL, hinokiol, and acacetin had significant inhibitory effects on 5-hydroxy-eicosa-tetra-enoic acid (5-HETE) and leukotriene B₄ (LTB4) formations. And cell-free enzyme assays (5-lipoxygenase, leukotriene A₄-hydrolase, cyclooxgenase-2) demonstrated the potent inhibitory effects of the CHL, hinokiol and acacetin on 5-lipoxygenase (5-LOX). Then, the inhibitions of the CHL, hinokiol on NO biosynthesis and the inhibitions of the CHL, 8(14),15-pimaradien-3尾,18-diol, and hinokiol on TNF-伪 release were also confirmed in the RAW264.7 murine macrophages.

Conclusion

The data indicate that the inhibitory effects of the CHL and its components (hinokiol and acacetin) on 5-LOX contribute to the anti-inflammatory activity of LPO. Moreover, the CHL and its components also show beneficial effects on NO and TNF-伪 production. Consequently, these results provide a rationale for LPO's traditional applications in the treatment of inflammatory airway diseases.