Quercetin inhibits rhinovirus replication in vitro and in vivo

详细信息	查看全文 | 推荐本文 |

• 作者：Shyamala Ganesan^a ; Andrea N. Faris^a ; Adam T. Comstock^a ; Qiong Wang^a ; Suparna Nanua^a ; Marc B. Hershenson^a ; ^b ; Uma S. Sajjan^a ; ^{usajjan@umich.edu}
• 关键词：Infection ; Translation ; Transcription ; Inflammation
• 刊名：Antiviral Research
• 出版年：2012
• 期刊代码：7_01663542
• 类别：imm
• 出版时间：June, 2012
• 卷：94
• 期：3
• 页码：258-271
• 文件大小：1721 K

摘要

Rhinovirus (RV), which is responsible for the majority of common colds, also causes exacerbations in patients with asthma and chronic obstructive pulmonary disease. So far, there are no drugs available for treatment of rhinovirus infection. We examined the effect of quercetin, a plant flavanol on RV infection in vitro and in vivo. Pretreatment of airway epithelial cells with quercetin decreased Akt phosphosphorylation, viral endocytosis and IL-8 responses. Addition of quercetin 6 h after RV infection (after viral endocytosis) reduced viral load, IL-8 and IFN responses in airway epithelial cells. This was associated with decreased levels of negative and positive strand viral RNA, and RV capsid protein, abrogation of RV-induced eIF4GI cleavage and increased phosphorylation of eIF2伪. In mice infected with RV, quercetin treatment decreased viral replication as well as expression of chemokines and cytokines. Quercetin treatment also attenuated RV-induced airway cholinergic hyperresponsiveness. Together, our results suggest that quercetin inhibits RV endocytosis and replication in airway epithelial cells at multiple stages of the RV life cycle. Quercetin also decreases expression of pro-inflammatory cytokines and improves lung function in RV-infected mice. Based on these observations, further studies examining the potential benefits of quercetin in the prevention and treatment of RV infection are warranted.