Ascorbic acid protects the diaphragm muscle against myonecrosis in mdx mice

详细信息	查看全文 | 推荐本文 |

• 作者：Erika Tonon ; M.Sc. ; Renato Ferretti ; M.Sc. ; Jean Hideki Shiratori ; B.Sc. ; Humberto Santo Neto ; Ph.D. ; Maria Julia Marques ; Ph.D. ; Elaine Minatel ; Ph.D. ; ^{minatel@unicamp.br}
• 关键词：Mdx mice ; Diaphragm muscle ; Ascorbic acid ; Oxidative stress ; Tumor necrosis factor-伪
• 刊名：Nutrition
• 出版年：2012
• 期刊代码：81_08999007
• 类别：abs
• 出版时间：June, 2012
• 卷：28
• 期：6
• 页码：686-690
• 文件大小：346 K

摘要

Objective

Oxidative stress contributes to myonecrosis in the dystrophin-deficient fibers of m>mdxm> mice and in Duchenne鈥檚 muscular dystrophy. We examined the effects of ascorbic acid (AA), an antioxidant and free radical scavenger, on the dystrophic diaphragm muscle.

Methods

m>Mdxm> mice (14 d old) received AA for 14 d. Control m>mdxm> mice received saline. The muscle damage was visualized by the penetration of Evans blue dye into myofibers and the extent of inflammation was assessed by histologic analysis. Creatine kinase levels were measured for the biochemical evaluation of muscle fiber degeneration. The levels of tumor necrosis factor-伪 (a聽proinflammatory cytokine) and 4-hydroxynonenal (a marker of lipid peroxidation) were analyzed by immunoblotting.

Results

Ascorbic acid decreased creatine kinase levels, myonecrosis, inflammation, and the levels of tumor necrosis factor-伪 and 4-hydroxynonenal.

Conclusion

The present results suggest that AA plays a protective role in dystrophic muscle degeneration, possibly by decreasing reactive oxygen species, and support further investigations of AA as a potential therapy for dystrophinopathies.