Electrochemical immunosensor based on mesoporous nanocomposites and HRP-functionalized nanoparticles bioconjugates for sensitivity enhanced detection of diethylstilbestrol

详细信息	查看全文 | 推荐本文 |

• 作者：Su Liu^a  ; [Author Vitae] ; Qing Lin^b  ; [Author Vitae] ; Xiuming Zhang^b  ; [Author Vitae] ; Xiaorui He^b  ; [Author Vitae] ; Xianrong Xing^b  ; [Author Vitae] ; Wenjing Lian^b  ; [Author Vitae] ; Jie Li^a  ; [Author Vitae] ; Min Cui^b  ; [Author Vitae] ; Jiadong Huang^c ; ^{chm_huangjd@ujn.edu.cn}  ; [Author Vitae]
• 关键词：Mesoporous silica&ndash ; gold nanoparticles&ndash ; multiwall carbon nanotubes nanocomposites ; Horseradish peroxidase&ndash ; antibody&ndash ; Prussian blue&ndash ; multiwall carbon nanotubes bioconjugates ; Electrochemical immunosensor ; Diethylstilbestrol detectio
• 刊名：Sensors and Actuators B ; Chemical
• 出版年：2012
• 期刊代码：161_09254005
• 类别：et
• 出版时间：20 May, 2012
• 卷：166-167
• 期：Complete
• 页码：562-568
• 文件大小：756 K

摘要

An ultrasensitive electrochemical immunosensor based on mesoporous silica-gold nanoparticles-multiwall carbon nanotubes (MSN-GNPs-MWCNTs) nanocomposites and horseradish peroxidase-antibody-Prussian blue-multiwall carbon nanotubes (HRP-Ab-GNPs-PB-MWCNTs) bioconjugates was developed for the detection of diethylstilbestrol (DES). MSN-GNPs-MWCNTs nanocomposites as immobilization matrix were used to enhance the electroactivity and stability of the electrode. HRP-Ab-GNPs-PB-MWCNTs bioconjugates as label were used to improve catalytic activity for hydrogen reduction of the electrode. Meanwhile, HRP-Ab-GNPs-PB-MWCNTs were used as the second signal amplification strategy for the detection of DES in the sandwich immunoreaction. Under the optimized conditions, a calibration plot for DES was obtained with a linear range between 3.3 脳 10^鈭? mg/mL and 4.5 脳 10^鈭? mg/mL (r = 0.996). The detection limit was 1.2 脳 10^鈭? mg/mL (S/N = 3). The proposed electrochemical immunosensor has excellent selectivity, good reproducibility and long-term stability. The total time taken for the assay was 7 min. The electrochemical immunosensor was examined in real samples for the analysis of DES. A good recovery in the range of 92-107%was obtained in milk and pork samples.