Highly stable electrochemical immunosensor for carcinoembryonic antigen

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• 作者：Xiaobin Sun ; Zhanfang Ma ; ^{mazhanfang@yahoo.com}
• 关键词：Carcinoembryonic antigen ; Electrochemical immunosensor ; Gold nanoparticles ; CAu bond
• 刊名：Biosensors and Bioelectronics
• 出版年：2012
• 期刊代码：12_09565663
• 类别：ch
• 出版时间：15 May, 2012
• 卷：35
• 期：1
• 页码：470-474
• 文件大小：485 K

摘要

The long-term stability of sensing interfaces is an important issue in biosensor fabrication. A novel stable gold nanoparticle (AuNP)-modified glassy carbon (GC) electrode interface (GC-Ph-AuNP)-based biosensor for detecting carcinoembryonic antigen (CEA) was developed. GC electrodes were modified with 1,4-phenylenediamine to form a stable layer, and then AuNPs were bound onto the GC electrodes through CAu bonds. Anti-CEA was directly adsorbed on AuNPs fixed on the GC electrode. The linear range of the immunosensor was from 10 fg to 100 ng mL^鈭? with a detection limit of 3 fg mL^鈭? (S/N = 3). The current of the immunosensor was increased by 4%after one month. The GC-Ph-AuNP immunosensor showed high sensitivity, a wide linear range, low detection limit, and good selectivity and stability. The immobilization method of the immunosensor could be widely applied to construct other immunosensors.