An ultrasensitive electrochemical immunosensor for the detection of salbutamol based on Pd@SBA-15 and ionic liquid

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• 作者：Zhentao Cui ; Yanyan Cai ; Dan Wu ; Haiqin Yu ; Yan Li ; Kexia Mao ; Huan Wang ; Haixia Fan ; Qin Wei ; ^{sdjndxwq@163.com} ; Bin Du ; ^{dubin61@gmail.com}
• 关键词：Sandwich-type immunosensor ; Salbutamol antigen ; Graphene sheets ; Pd@SBA-15 ; Ionic liquid
• 刊名：Electrochimica Acta
• 出版年：2012
• 期刊代码：30_00134686
• 类别：ch
• 出版时间：1 May, 2012
• 卷：69
• 期：Complete
• 页码：79-85
• 文件大小：1193 K

摘要

A sandwich-type electrochemical immunosensor for the ultrasensitive detection of the salbutamol (SAL) antigen based on sodium dodecylbenzene sulfonate-functionalized graphene sheets (SDBS-GS), Pd nanoparticles in functionalized SBA-15 (Pd@SBA-15) and ionic liquid (IL) is described. The primary SAL antibody (Ab₁) can be covalently attached to the sulfonic groups of SDBS-GS through the amines of Ab₁ with an acyl chloride cross-linking reaction. Pd@SBA-15 was prepared by adsorption of H₂PdCl₄ onto the SBA-15 surface; the adsorbed H₂PdCl₄ was then reduced to Pd nanoparticles with sodium borohydride. Pd@SBA-15 was conjugated to the secondary SAL antibody (Ab₂) through glutaraldehyde. IL was added to the mixture of Pd@SBA-15 and Ab₂ to promote electron transport. The synergistic effect between IL and Pd@SBA-15 retained the bioactivity of Ab₂. The sensitivity of the sandwich-type immunosensor using Pd@SBA-15/Ab₂/IL for the detection of SAL was much higher than those that used either SBA-15/Ab₂ or Pd@SBA-15/Ab₂. Under optimal conditions, the electrochemical immunosensor exhibited a wide working range from 0.02 to 15.0 ng/mL with a detection limit of 7 pg/mL. The precision, reproducibility and stability of the immunosensor were acceptable. The proposed strategy could be easily extended to fabricate immunosensors for other tumor markers.