Enhancement of the immunogenicity of an alphavirus replicon-based DNA vaccine against classical swine fever by electroporation and coinjection with a plasmid expressing porcine interleukin 2

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• 作者：Da-Yong Tian^a ; ¹ ; Yuan Sun^a ; ¹ ; Sing Fai Wai^b ; Fuk Ki Lee^b ; Qi-Lin Meng^a ; Kar Man Suen^b ; Nan Wang^a ; Wen Han^a ; Su Li^a ; Yong-Feng Li^a ; Dan Li^a ; Li-Jun Ling^a ; Ya-Jin Liao^a ; Hua-Ji Qiu^a ; ^{huajiqiu@hvri.ac.cn} ; ^{qiuhuaji@163.com}
• 关键词：Classical swine fever ; Alphavirus replicon ; Electroporation ; Interleukin 2
• 刊名：Vaccine
• 出版年：2012
• 期刊代码：89_0264410x
• 类别：imm
• 出版时间：21 May, 2012
• 卷：30
• 期：24
• 页码：3587-3594
• 文件大小：657 K

摘要

Alphavirus replicon-based DNA vaccines have emerged as a promising approach to generation of antigen-specific immune responses. However, due to their low immunogenicity, there is a need for other approaches to enhance the vaccine potency. In this study, electroporation (EP) and a plasmid expressing porcine interleukin 2 (IL-2) were used to improve the immunogenicity of an alphavirus replicon-based DNA vaccine pSFV1CS-E2 against classical swine fever (CSF). Pigs were immunized with pSFV1CS-E2 alone or together with IL-2 by EP or by simple intramuscular injection. The results showed that EP combined with IL-2 resulted in marked enhancement of E2-specific antibody responses. Moreover, CSFV-specific lymphocyte proliferation, IFN-纬 and IL-4 responses were increased significantly in the pSFV1CS-E2+IL-2/EP group. Pigs immunized with pSFV1CS-E2 plus IL-2 by EP were completely protected from lethal challenge, which is comparable to the sterilizing immunity and full protection offered by the live attenuated vaccine C-strain and in contrast with the incomplete protection conferred by pSFV1CS-E2 without or with IL-2 or EP alone, as demonstrated by the presence of pathological changes or/and viral loads. We conclude that EP in combination with IL-2 can significantly improve the immunogenicity of the plasmid DNA vaccine.