Analysis of 8-hydroxy-2鈥?deoxyguanosine in human urine using hydrophilic interaction chromatography with tandem mass spectrometry

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• 作者：Chiemi Hosozumi^a ; Akira Toriba^a ; ^{toriba@p.kanazawa-u.ac.jp} ; Thanyarat Chuesaard^a ; Takayuki Kameda^a ; Ning Tang^b ; Kazuichi Hayakawa^a
• 关键词：Hydrophilic interaction chromatography ; Tandem mass spectrometry ; 8-Hydroxy-2&prime ; -deoxyguanosine ; Urine ; Oxidative stress
• 刊名：Journal of Chromatography B ; Analytical Technologies in the Biomedical and Life Sciences
• 出版年：2012
• 期刊代码：124_15700232
• 类别：ch
• 出版时间：15 April, 2012
• 卷：893-894
• 期：Complete
• 页码：173-176
• 文件大小：256 K

摘要

Urinary 8-hydroxy-2鈥?deoxyguanosine (8-OHdG) is a widely used noninvasive biomarker of oxidative stress. A selective, sensitive and rapid method for determining 8-OHdG in human urine was developed using hydrophilic interaction chromatography-tandem mass spectrometry (HILIC-MS/MS) with electrospray ionization. 8-OHdG and isotopically labeled 8-OHdG (internal standard) were separated on a HILIC column with a mobile phase of 10 mM ammonium acetate: acetonitrile (1:9, v/v) within 10 min and detected by using a positive electrospray ionization interface under the selected reaction monitoring mode. The detection limits of 8-OHdG (corresponding to a signal-to-noise ratio of 3) for the HILIC-MS/MS system and the conventional method using a reversed-phase column with MS/MS were 1.0 and 26.0 fmol/injection, respectively. The proposed method makes it possible to monitor the basal level of urinary 8-OHdG from non-exposed healthy subjects and can be used for large-scale human studies.