Fabrication of gelatin/calcium phosphate composite nanofibrous membranes by biomimetic mineralization

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• 作者：Mi Ok Choi ; Young-Jin Kim ; ^{yjkim@cu.ac.kr}
• 关键词：Biomimetic mineralization ; Calcium phosphate ; Electrospinning ; Gelatin
• 刊名：International Journal of Biological Macromolecules
• 出版年：2012
• 期刊代码：62_01418130
• 类别：abs
• 出版时间：1 June, 2012
• 卷：50
• 期：5
• 页码：1188-1194
• 文件大小：1638 K

摘要

Based on the principles of biomimetic mineralization, biocomposite nanofibrous membranes were fabricated by the growth of CaP crystals on electrospun gelatin nanofibers to mimic both the physical architecture and chemical composition of natural bone ECM. Plenty more CaP crystals formed on the nanofibrous membrane containing Ca²⁺ ion precursors, in which these crystals were also observed on the inner side of membrane. The release rate of Ca²⁺ ion precursors from the nanofibrous membrane was slower than that of ion precursors, suggesting the existence of more strong intermolecular interaction between gelatin and Ca²⁺ ions. ATR-FTIR and XRD results clearly revealed the formation of CaP crystals mixed with apatite and CaCO₃, or apatite and TCP on the membranes. The Ca/P molar ratio of crystals obtained from the XPS data was 2.03 and 1.60, which depended on the mineralization conditions. Higher amount of CaP crystals significantly accelerated the deposit rate of bone-like apatite on the surface of composite membrane, meaning to the improved in vivo bone bioactivity.