- 作者：Matthew J. Price ; MD鈦?/sup> ; &dagger ; ; price.matthew@scrippshealth.org ; Sarah S. Murray ; PhD&dagger ; ; Dominick J. Angiolillo ; MD ; PhD&Dagger ; ; Elizabeth Lillie ; PhD&dagger ; ; Erin N. Smith ; PhD§ ; ; Rebecca L. Tisch ; BS&dagger ; ; Nicholas J. Schork ; PhD&dagger ; ; Paul S. Teirstein ; MD鈦?/sup> ; Eric J. Topol ; MD鈦?/sup> ; &dagger ; ; GIFT Investigators
- 关键词：clopidogrel ; CYP2C19 ; genotype ; platelet function testing ; thienipyridine
- 刊名：Journal of the American College of Cardiology
- 出版年：2012
- 期刊代码：201_07351097
- 类别：med
- 出版时间：29 May, 2012
- 卷：59
- 期：22
- 页码：1928-1937
- 文件大小：525 K
Objectives
This study sought to evaluate the influence of single nucleotide polymorphisms (SNPs) on the pharmacodynamic effect of high- or standard-dose clopidogrel after percutaneous coronary intervention (PCI).Background
There is a lack of prospective, multicenter data regarding the effect of different genetic variants on clopidogrel pharmacodynamics over time in patients undergoing PCI.
Methods
The GRAVITAS (Gauging Responsiveness with A VerifyNow assay-Impact on Thrombosis And Safety) trial screened patients with platelet function testing after PCI and randomly assigned those with high on-treatment reactivity (OTR) to either high- or standard-dose clopidogrel; a cohort of patients without high OTR were also followed. DNA samples obtained from 1,028 patients were genotyped for 41 SNPs in 17 genes related to platelet reactivity. After adjusting for clinical characteristics, the associations between the SNPs and OTR using linear regression were evaluated.
Results
CYP2C19*2 was significantly associated with OTR at 12 to 24 h (R2 = 0.07, p = 2.2 脳 10鈭?5), 30 days (R2 = 0.10, p = 1.3 脳 10鈭?), and 6 months after PCI (R2 = 0.07, p = 1.9 脳 10鈭?1), whereas PON1, ABCB1 3435 C鈫扵, and other candidate SNPs were not. Carriers of 1 and 2 reduced-function CYP2C19 alleles were significantly more likely to display persistently high OTR at 30 days and 6 months, irrespective of treatment assignment. The portion of the risk of persistently high OTR at 30 days attributable to reduced-function CYP2C19 allele carriage was 5.2%in the patients randomly assigned to high-dose clopidogrel.
Conclusions
CYP2C19, but not PON1 or ABCB1, is a significant determinant of the pharmacodynamic effects of clopidogrel, both early and late after PCI. In patients with high OTR identified by platelet function testing, the CYP2C19 genotype provides limited incremental information regarding the risk of persistently high reactivity with clopidogrel 150-mg maintenance dosing. (Genotype Information and Functional Testing Study [GIFT]; )