Purification, molecular cloning and functional characterization of HelaTx1 (Heterometrus laoticus): The first member of a new 魏-KTX subfamily

详细信息	查看全文 | 推荐本文 |

• 作者：Thomas Vandendriessche^a ; Ivan Kopljar^b ; David Paul Jenkins^c ; Elia Diego-Garcia^a ; Yousra Abdel-Mottaleb^a ; Elke Vermassen^a ; Elke Clynen^d ; ^e ; Liliane Schoofs^d ; Heike Wulff^c ; Dirk Snyders^b ; Jan Tytgat^a ; ^{Jan.Tytgat@pharm.kuleuven.be}
• 关键词：Scorpion toxin ; Voltage-gated potassium channel ; Electrophysiology ; Patch cl cDNA ; Heterometrus laoticus
• 刊名：Biochemical Pharmacology
• 出版年：2012
• 期刊代码：2_00062952
• 类别：pha
• 出版时间：1 May, 2012
• 卷：83
• 期：9
• 页码：1307-1317
• 文件大小：1391 K

摘要

Given their medical importance, most attention has been paid toward the venom composition of scorpions of the Buthidae family. Nevertheless, research has shown that the venom of scorpions of other families is also a remarkable source of unique peptidyl toxins. The 魏-KTx family of voltage-gated potassium channel (VGPC) scorpion toxins is hereof an example. From the telson of the scorpion Heterometrus laoticus (Scorpionidae), a peptide, HelaTx1, with unique primary sequence was purified through HPLC and sequenced by Edman degradation. Based on the amino acid sequence, the peptide could be cloned and the cDNA sequence revealed. HelaTx1 was chemically synthesized and functionally characterized on VGPCs of the Shaker-related, Shab-related, Shaw-related and Shal-related subfamilies. Furthermore, the toxin was also tested on small- and intermediate conductance Ca²⁺-activated K⁺ channels. From the channels studied, K_v1.1 and K_v1.6 were found to be the most sensitive (K_v1.1 EC₅₀ = 9.9 卤 1.6 渭M). The toxin did not alter the activation of the channels. Competition experiments with TEA showed that the toxin is a pore blocker. Mutational studies showed that the residues E353 and Y379 in the pore of K_v1.1 act as major interaction points for binding of the toxin. Given the amino acid sequence, the predicted secondary structure and the biological activity on VGPCs, HelaTx1 should be included in the 魏-KTX family. Based on a phylogenetic study, we rearranged this family of VGPC toxins into five subfamilies and suggest that HelaTx1 is the first member of the new 魏-KTx5 subfamily.