Anti-inflammatory effects of Lafoensia pacari and ellagic acid in a murine model of asthma
- 作者：Alex ; re P. Rogerio ; Caroline Fontanari ; É ; rica Borducchi ; Alex ; re C. Keller ; Momtchilo Russo ; Edson G. Soares ; Deijanira A. Albuquerque ; Lú ; cia H. Faccioli
- 关键词：Ellagic acid ; Asthma ; Eosinophils ; (Lafoensia pacari)
- 刊名：European Journal of Pharmacology
- 出版年：2008
- 期刊代码：24_00142999
- 类别：neu
- 出版时间：2 February 2008
- 卷：580
- 期：1-2
- 页码：262-270
- 文件大小：1361 K
摘要
We have shown that the ethanolic extract of Lafoensia pacari inhibits eosinophilic inflammation induced by Toxocara canis infection, and that ellagic acid is the secondary metabolite responsible for the anti-eosinophilic activity seen in a model of β-glucan peritonitis. In the present study, we investigated the preventive and curative effects of L. pacari extract and ellagic acid on allergic lung inflammation using a murine model of ovalbumin-induced asthma. In bronchoalveolar lavage fluid, preventive (22-day) treatment with L. pacari (200 mg/kg) and ellagic acid (10 mg/kg) inhibited neutrophil counts (by 75%and 57%) and eosinophil counts (by 78%and 68%). L. pacari reduced IL-4 and IL-13 levels (by 67%and 73%), whereas ellagic acid reduced IL-4, IL-5 and IL-13 (by 67%, 88%and 85%). To investigate curative anti-inflammatory effects, we treated mice daily with ellagic acid (0.1, 1, or 10 mg/kg), also treating selected mice with L. pacari (200 mg/kg) from day 18 to day 22. The highest ellagic acid dose reduced neutrophil and eosinophil numbers (by 59%and 82%), inhibited IL-4, IL-5, and IL-13 (by 62%, 61%, and 49%). Neither L. pacari nor ellagic acid suppressed ovalbumin-induced airway hyperresponsiveness or cysteinyl leukotriene synthesis in lung homogenates. In mice treated with ellagic acid (10 mg/kg) or L. pacari (200 mg/kg) at 10 min after the second ovalbumin challenge, eosinophil numbers were 53%and 69%lower, respectively. Cytokine levels were unaffected by this treatment. L. pacari and ellagic acid are effective eosinophilic inflammation suppressors, suggesting a potential for treating allergies.