Drug eluting stents based on Poly(ethylene carbonate): Optimization of the stent coating process

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• 作者：Nadja Bege^a ; Sven Ole Steinmü ; ller^b ; Marc Kalinowski^c ; Regina Reul^a ; Sebastian Klaus^d ; Holger Petersen^e ; Catherine Curdy^e ; Jü ; rgen Janek^b ; Thomas Kissel^a ; ^{kissel@staff.uni-marburg.de}
• 关键词：DES ; PEC ; TOF-SIMS ; Spray-coating ; Late stent thrombosis ; Paclitaxel
• 刊名：European Journal of Pharmaceutics and Biopharmaceutics
• 出版年：2012
• 期刊代码：16_09396411
• 类别：pha
• 出版时间：April, 2012
• 卷：80
• 期：3
• 页码：562-570
• 文件大小：1589 K

摘要

First generation drug eluting stents (DES) show a fivefold higher risk of late stent thrombosis compared to bare metal stents. Therefore, new biodegradable and biocompatible polymers for stent coating are needed to reduce late stent thrombosis. In this study, a reproducible spray-coating process for stents coated with Poly(ethylene carbonate), PEC, and Paclitaxel was investigated. PEC is a biocompatible, thermoelastic polymer of high molecular weight. The surface degradation of PEC is triggered by superoxide anions produced by polymorphonuclear leukocytes and macrophages during inflammatory processes. Stents with different drug loading were reproducibly produced by a spray-coating apparatus. Confocal laser scanning micrographs of fluorescent dye loaded stents were made to investigate the film homogeneity. The abluminal stent site was loaded more than the luminal site, which is superior for DES. The deposition of the layers was confirmed by TOF-SIMS investigations. Referring to the stent surface, the drug loading is 0.32 渭g (卤0.05) (once coated), 0.53 渭g (卤0.11) (twice coated), or 0.73 渭g (卤0.06) (three times coated) Paclitaxel per mm² stent surface. The in vitro release mechanism during non-degradation conditions can be explained by diffusion-controlled drug release slightly influenced by swelling of PEC, revealing that 100%of the loaded Paclitaxel will be released via diffusion within 2 months. So, the in vivo release kinetic is a combination of diffusion-controlled drug release and degradation-controlled drug release depending on the presence or absence of superoxide anions and accordingly depending on the presence or absence of macrophages. We conclude that the specific release kinetics of PEC, its biocompatibility, and the favorable mechanical properties will be beneficial for a next generation drug eluting stent meriting further investigations under in vivo conditions.