CD45^lowc-Kit^high cells have hematopoietic properties in the mouse aorta-gonad-mesonephros region

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• 作者：Ikuo Nobuhisa^a ; ^b ; ^{nobuhisa.scr@mri.tmd.ac.jp} ; Shoutarou Yamasaki^b ; Ahmed Ramadan^a ; ^b ; Tetsuya Taga^a ; ^b ; ^{taga.scr@mri.tmd.ac.jp}
• 关键词：Hematopoiesis ; Mouse embryo ; AGM ; CD45 ; c-Kit
• 刊名：Experimental Cell Research
• 出版年：2012
• 期刊代码：292_00144827
• 类别：med
• 出版时间：1 April, 2012
• 卷：318
• 期：6
• 页码：705-715
• 文件大小：2013 K

摘要

Long-term reconstituting hematopoietic stem cells first arise from the aorta of the aorta-gonad-mesonephros (AGM) region in a mouse embryo. We have previously reported that in cultures of the dispersed AGM region, CD45^lowc-Kit⁺ cells possess the ability to reconstitute multilineage hematopoietic cells, but investigations are needed to show that this is not a cultured artifact and to clarify when and how this population is present. Based on the expression profile of CD45 and c-Kit in freshly dissociated AGM cells from embryonic day 9.5 (E9.5) to E12.5 and aorta cells in the AGM from E13.5 to E15.5, we defined six cell populations (CD45^{鈭?/sup>c-Kit鈭?/sup>, CD45鈭?/sup>c-Kitlow, CD45鈭?/sup>c-Kithigh, CD45lowc-Kithigh, CD45highc-Kithigh, and CD45highc-Kitvery low). Among these six populations, CD45lowc-Kithigh cells were most able to form hematopoietic cell colonies, but their ability decreased after E11.5 and was undetectable at E13.5 and later. The CD45lowc-Kithigh cells showed multipotency in vitro. We demonstrated further enrichment of hematopoietic activity in the Hoechst dye-effluxing side population among the CD45lowc-Kithigh cells. Here, we determined that CD45lowc-Kithigh cells arise from the lateral plate mesoderm using embryonic stem cell-derived differentiation system. In conclusion, CD45lowc-Kithigh cells are the major hematopoietic cells of mouse AGM.}