Dynamics of Setdb1 expression in early mouse development

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• 作者：Sunwha Cho^a ; ^b ; ^{swcho@kribb.re.kr} ; Jung Sun Park^a ; ^{jspark@kribb.re.kr} ; Sujin Kwon^a ; ^b ; ^{appie18@hanmail.net} ; Yong-Kook Kang^a ; ^b ; ^{ykkang@kribb.re.kr}
• 关键词：Setdb1/Eset ; Mouse embryo ; Histone methylation ; Blastocyst outgrowth ; Embryonic stem cell
• 刊名：Gene Expression Patterns
• 出版年：2012
• 期刊代码：26_1567133x
• 类别：neu
• 出版时间：May-June, 2012
• 卷：12
• 期：5-6
• 页码：213-218
• 文件大小：985 K

摘要

Setdb1/Eset, a histone lysine methyltransferase, is recruited by various transcription factors to modify local chromatin. The observation that Setdb1-null blastocysts fail to produce epiblast-lineage cells suggests a role for Setdb1 in generating mouse embryonic stem cells (mESCs). When examined in mouse zygotes, Setdb1 proteins appeared as dots at perinucleolar rims of pronuclei, with the dot-shaped signals more prominent in male pronuclei. Setdb1 signals were observed diffusely in the nucleus from the two-cell stage onward and, by the blastocyst, took a punctate form, away from nucleolus. Such varying expression patterns suggest its involvement in diverse biological processes at preimplantation stage. Setdb1 appeared in Oct4-positive cells of inner-cell-mass origin but not in trophectoderm-lineage cells in blastocyst outgrowths. Setdb1 co-immunoprecipitated with Oct4 in mESCs, and Setdb1 expression was markedly reduced upon retinoic acid-induced differentiation. These observations suggest that Setdb1 has an important role in maintaining the self-renewal of mESCs through collaboration with Oct4.