Arginine decreases Cryptosporidium parvum infection in undernourished suckling mice involving nitric oxide synthase and arginase

详细信息	查看全文 | 推荐本文 |

• 作者：Ibraim C. Castro ; M.D.^a ; ^c ; Bruna B. Oliveira ; M.D.^a ; ^c ; Jacek J. Slowikowski ; M.Sc.^a ; Bruna P. Coutinho ; M.D.^a ; ^c ; Francisco Jú ; lio W.S. Siqueira ; M.D.^a ; ^c ; Lourrany B. Costa^a ; ^c ; Jesus Emmanuel Sevilleja ; M.D.^a ; Camila A. Almeida ; D.V.M.^b ; Aldo A.M. Lima ; M.D. ; Ph.D.^a ; ^c ; ^d ; Cirle A. Warren ; M.D.^a ; ^c ; Reinaldo B. Oriá ; D.V.M. ; Ph.D.^a ; ^b ; ^c ; ^{rbo5u@virginia.edu} ; Richard L. Guerrant ; M.D.^a ; ^c
• 关键词：Cryptosporidium parvum ; Diarrhea ; Malnutrition ; Arginine ; Growing mice
• 刊名：Nutrition
• 出版年：2012
• 期刊代码：81_08999007
• 类别：abs
• 出版时间：June, 2012
• 卷：28
• 期：6
• 页码：678-685
• 文件大小：1330 K

摘要

Objective

This study investigated the role of L-arginine supplementation to undernourished and Cryptosporidium parvum-infected suckling mice.

Methods

The following regimens were initiated on the fourth day of life and injected subcutaneously daily. The C. parvum-infected controls received L-arginine (200 mmol/L) or phosphate buffered saline. The L-arginine-treated mice were grouped to receive NG-nitro-arginine methyl ester (L-NAME) (20 mmol/L) or phosphate buffered saline. The infected mice received orally 10⁶ excysted C. parvum oocysts on day 6 and were euthanized on day 14 at the infection peak.

Results

L-arginine improved weight gain compared with the untreated infected controls. L-NAME profoundly impaired body weight gain compared with all other groups. Cryptosporidiosis was associated with ileal crypt hyperplasia, villus blunting, and inflammation. L-arginine improved mucosal histology after the infection. L-NAME abrogated these arginine-induced improvements. The infected control mice showed an intense arginase expression, which was even greater with L-NAME. L-arginine decreased the parasite burden, an effect that was reversed by L-NAME. Cryptosporidium parvum infection increased urine NO₃^{鈭?/sup>/NO₂鈭?/sup> concentrations compared with the uninfected controls, which was increased by L-arginine supplementation, an effect that was also reversed by L-NAME.}

Conclusion

These findings show a protective role of L-arginine during C. parvum infection in undernourished mice, with involvement of arginase I and nitric oxide synthase enzymatic actions.