Redesigning the single-chain variant of the restriction endonuclease PvuII by circular permutation

详细信息	查看全文 | 推荐本文 |

• 作者：Benno Schierling ; ^{benno.schierling@chemie.bio.uni-giessen.de} ; Wolfgang Wende ; Alfred Pingoud
• 关键词：CP ; circular permutation ; scPvuII ; single-chain variant of PvuII
• 出版年：2012
• 期刊代码：70_00145793
• 类别：bio
• 出版时间：12 June, 2012
• 卷：586
• 期：12
• 页码：1736-1741
• 文件大小：1133 K

摘要

The restriction endonuclease PvuII has been introduced as a sequence-specific cleavage module in highly-specific nucleases for gene targeting. Here, a structural reorganization of the single-chain variant of PvuII (scPvuII) was performed by circular permutation as a proof-of-concept in order to find out whether the relocated, new termini next to structural elements important for DNA recognition and catalysis could be used for the fusion with other regulatory protein domains. Three circularly permuted variants of scPvuII were obtained that all maintain the specific endonucleolytic activity of scPvuII.