Bacterial cell envelopes (ghosts) but not S-layers activate human endothelial cells (HUVECs) through sCD14 and LBP mechanism
- 作者：Fü ; rst-Ladani ; Shayesteh ; Redl ; Heinz ; Haslberger ; Alexander ; Lubitz ; Werner ; Messner ; Paul ; et. al.
- 关键词：Endotoxicity ; Bacterial ghosts ; S-layers ; Cytokines ; HUVEC
- 刊名：Vaccine
- 出版年：1999
- 期刊代码：89_0264410x
- 类别：imm
- 出版时间：October 14, 1999
- 卷：18
- 期：5-6
- 页码：440-448
- 文件大小：181 K
摘要
Bacterial cell-envelopes (called ghosts) and surface layers (S-layers) are discussed to be used as vaccines and/or adjuvants, consequently it is necessary to find out which immunomodulatory mediators are induced in human cells. The present work focuses on the effects of ghosts (Escherichia coli O26:B6), S-layers (Bacillus stearothermophilus) in comparison with LPS and antibiotic-inactivated whole bacteria (E. coli O26:B6) on human umbilical vein endothelial cells (HUVEC) with regard to the release of interleukin 6 (IL-6) and the expression of surface E-selectin and the role of lipopolysaccharide binding protein (LBP), soluble CD14 (sCD14) and serum for this activation.