Monoubiquitination of pro-amphiregulin regulates its endocytosis and ectodomain shedding

详细信息	查看全文 | 推荐本文 |

• 作者：Shinji Fukuda^a ; ^{sfukuda@m.ehime-u.ac.jp} ; Hisayo Nishida-Fukuda^a ; Hironao Nakayama^a ; Hirofumi Inoue^a ; ^b ; ^c ; Shigeki Higashiyama^a ; ^c ; ^{shigeki@m.ehime-u.ac.jp}
• 关键词：Amphiregulin ; Epidermal growth factor receptor ; Ubiquitination ; Ectodomain shedding
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2012
• 期刊代码：159_0006291x
• 类别：bio
• 出版时间：6 April, 2012
• 卷：420
• 期：2
• 页码：315-320
• 文件大小：884 K

摘要

All members of epidermal growth factor (EGF) family are expressed as transmembrane precursors on cell surfaces and then proteolytically converted to soluble ligands for EGF receptor (EGFR) by a disintegrin and metalloproteases (ADAMs). As enzyme-substrate complex formation is essential for this 鈥渆ctodomain shedding鈥? alteration of cell surface retention could affect their physical interaction with ADAMs and eventually contribute to shedding efficiency. Here, we showed that monoubiquitination of pro-amphiregulin (pro-AREG, an EGFR ligand) accelerated its half-life on cell surface. Monoubiquitination occurred at lysine 240 of pro-AREG as the primary acceptor site. Using a chimeric protein of pro-AREG and a monomeric ubiquitin mutant (pro-AREGmUb), immunocytochemical analysis and a cell surface biotinylation assay revealed that a significant portion of pro-AREGmUb was expressed on the cell surface, immediately endocytosed, and predominantly localized to early endosomes. Importantly, ectodomain shedding of pro-AREGmUb induced by tetradecanoyl phorbol acetate was significantly reduced in comparison to wild-type pro-AREG. These results suggested that pro-AREG monoubiquitination and the subsequent trafficking to intracellular organelles is a novel shedding regulatory mechanism that contributes to the secretion of EGFR ligands in growth factor signaling.