Dolichos biflorus agglutinin-binding site expression in basal keratinocytes is associated with cell differentiation
- 作者：Dvorá ; nková ; Barbora ; Motlí ; k ; Jan ; Holí ; ková ; Zuzana ; et. al.
- 关键词：Epidermis ; Epidermal stem cell ; Differentiation ; β ; 1 integrin ; Golgi complex ; Dolichos biflorus agglutinin
- 刊名：Biology of the Cell
- 出版年：2002
- 期刊代码：11_02484900
- 类别：imm
- 出版时间：October 1, 2002
- 卷：94
- 期：6
- 页码：365-373
- 文件大小：521 K
摘要
A basal layer of squamous epithelia such as epidermis contains stem cells, transit amplifying cells as well as postmitotic differentiating cells. A detailed knowledge of the transition among these cell types in the course of epidermal renewal is important. It would help in better understanding of many pathological processes, including cancer, and in employment of epidermal cells for therapeutic purposes. In this study we analyzed the possible role of Dolichos biflorus agglutinin (DBA)-reactive α-N-acetylgalactosamine glycosylation in behavior of the human epidermal basal cells under in vivo and in vitro conditions. The data received from porcine epidermis were also included. Part of basal cells was positive for DBA-binding sites and these cells exhibited a lower presence of β1 integrin in their basal surface connected to the basement membrane. The perinuclear Golgi-like accumulation of β1 integrin was observed in some cultured keratinocytes. The co-localization of integrin with DBA-binding sites and 58 kDa protein suggests that α-N-acetylgalactosamine glycosylation could be related to β1 integrin retention in the endoplasmatic reticulum Golgi intermediate compartment (ERGIC) at the beginning of the secretory pathway. The lack of anchorage in culture elevated the number of DBA-binding site positive cells without significant influence on cell growth when cells isolated directly from epidermis were employed in study. Some role of DBA-reactive glycoligand expressions in a suprabasal movement of differentiated basal cells can be hypothesized.