Spatial gene expression in the T-stage mouse metanephros
- 作者：Georgina Caruana ; Luise Cullen-McEwen ; Amy L. Nelson ; Xenia Kostoulias ; Kyra Woods ; Brooke Gardiner ; Melissa J. Davis ; Darrin F. Taylor ; Rohan D. Teasdale ; Sean M. Grimmond et al.
- 关键词：Metanephros ; Ureteric epithelium ; Ureteric tip cells ; Ureteric trunk cells ; Metanephric mesenchyme ; Branching morphogenesis ; Microarray ; sim-1 ; Tacstd1 ; Crlf-1 ; CSPG2/versican ; CV-2/BMPER ; BMP4
- 刊名：Gene Expression Patterns
- 出版年：2006
- 期刊代码：26_1567133x
- 类别：neu
- 出版时间：2 October 2006
- 卷：6
- 期：8
- 页码：807-825
- 文件大小：689 K
摘要
The E11.5 mouse metanephros is comprised of a T-stage ureteric epithelial tubule sub-divided into tip and trunk cells surrounded by metanephric mesenchyme (MM). Tip cells are induced to undergo branching morphogenesis by the MM. In contrast, signals within the mesenchyme surrounding the trunk prevent ectopic branching of this region. In order to identify novel genes involved in the molecular regulation of branching morphogenesis we compared the gene expression profiles of isolated tip, trunk and MM cells using Compugen mouse long oligo microarrays. We identified genes enriched in the tip epithelium, sim-1, Arg2, Tacstd1, Crlf-1 and BMP7; genes enriched in the trunk epithelium, Innp1, Itm2b, Mkrn1, SPARC, Emu2 and Gsta3 and genes spatially restricted to the mesenchyme surrounding the trunk, CSPG2 and CV-2, with overlapping and complimentary expression to BMP4, respectively. This study has identified genes spatially expressed in regions of the developing kidney involved in branching morphogenesis, nephrogenesis and the development of the collecting duct system, calyces, renal pelvis and ureter.