Evidence for the porcine origin of equine rotavirus strain H-1
- 作者：Souvik Ghosh ; souvikrota@gmail.com ; souvik8@rediffmail.com ; Tsuzumi Shintani ; Nobumichi Kobayashi
- 关键词：Equine rotavirus ; Whole genomic analysis ; Porcine origin ; Interspecies transmission
- 刊名：Veterinary Microbiology
- 出版年：2012
- 期刊代码：106_03781135
- 类别：abs
- 出版时间：17 August, 2012
- 卷：158
- 期：3-4
- 页码：410-414
- 文件大小：602 K
摘要
Equine group A rotavirus (RVA) strain H-1 (RVA/Horse-tc/GBR/H-1/1975/G5P9[7]) was found to have VP4, VP6-7, NSP1 and NSP4 genes of porcine origin. In order to obtain conclusive information on the exact origin and evolution of this unusual equine strain, the remaining six genes (VP1-3, NSP2-3 and NSP5 genes) of strain H-1 were analyzed in the present study. By whole genomic analysis, strain H-1 exhibited a porcine RVA-like genotype constellation (G5-P[7]-I5-R1-C1-M1-A8-N1-T1-E1-H1), different from those of typical equine RVA strains. The VP2-3 and NSP2-3 genes of strain H-1 were found to originate from porcine RVAs. On the other hand, it was difficult to pinpoint the exact origin of the VP1 and NSP5 genes of strain H-1, though phylogenetically, these genes appeared to be possibly derived from porcine or Wa-like human strains. Taken together, at least nine (VP2-4, VP6-7 and NSP1-4 genes) of the 11 gene segments of strain H-1 were found to be of porcine origin, revealing a porcine RVA-like genetic backbone. Therefore, strain H-1 is likely a porcine RVA strain that was transmitted to horses.