Protection of pigs against genital Chlamydia trachomatis challenge by parenteral or mucosal DNA immunization

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• 作者：Katelijn Schautteet^a ; ^{Katelijn.Schautteet@UGent.be} ; Evelien De Clercq^a ; Yannick Jö ; nsson^a ; Stefanie Lagae^a ; Koen Chiers^b ; Eric Cox^c ; Daisy Vanrompay^a
• 关键词：DNA vaccine ; Chlamydia trachomatis and genital infection
• 刊名：Vaccine
• 出版年：2012
• 期刊代码：89_0264410x
• 类别：imm
• 出版时间：16 April, 2012
• 卷：30
• 期：18
• 页码：2869-2881
• 文件大小：1153 K

摘要

The current study evaluates combined aerosol-vaginal delivery of a MOMP-based Chlamydia trachomatis (serovar E) DNA vaccine in a pig genital challenge model. Most non-replicating antigens are rather poor mucosal immunogens in comparison to replicating antigens. Therefore, a mucosal administered DNA vaccine, which actually mimics a live vaccine, could be promising. Protection was promoted by plasmids encoding the porcine granulocyte macrophage-colony stimulating factor (pcDNA3.1zeo::GM-CSF), the Escherichia coli thermo-labile enterotoxin (LT) subunit A (plasmid PJV2004::LTa) and subunit B (plasmid PJV2005::LTb). Mucosal C. trachomatis DNA vaccination induced significant protection against genital C. trachomatis challenge although the infection could not be eradicated. Intradermal immunization was significantly less efficient in protecting experimentally infected pigs. Protection was correlated with efficient T cell priming and significantly higher serum IgA titers following primo vaccination.