Potato fiber protects the small intestinal wall against the toxic influence of acrylamide

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• 作者：Piotr Dobrowolski ; Ph.D.^a ; ^{piotr.dobrowolski@umcs.lublin.pl} ; Pauline Huet ; Ph.D.^b ; Patrik Karlsson ; Ph.D.^c ; Sune Eriksson ; Ph.D.^d ; Ewa Tomaszewska ; Ph.D.^e ; Antoni Gawron^a (Prof.) ; Stefan G. Pierzynowski^b ; ^f (Prof.)
• 关键词：Potato fiber ; Dietary fiber ; Acrylamide ; Small intestine ; Histomorphometry ; Mouse
• 刊名：Nutrition
• 出版年：2012
• 期刊代码：81_08999007
• 类别：abs
• 出版时间：April, 2012
• 卷：28
• 期：4
• 页码：428-435
• 文件大小：932 K

摘要

Objective

Acrylamide is a neurotoxic, genotoxic substance present in many commonly consumed food products and has been shown to have carcinogenic effects in rodents. The protective effects (if any) of potato fiber preparations, composed of cell wall material from potatoes, against the toxic influence of dietary acrylamide on the small intestinal wall were investigated.

Methods

Male mice of the BALB/c strain were used in the study. Acrylamide was administered to the mice in their drinking water (0.5 mg/kg of body weight per day) and one of two types of potato fiber preparations (heated or raw potato fiber preparation) was added to their feed (2%addition to their feed). Histomorphometry of the small intestinal wall, hemoglobin adducts of acrylamide, animal weight, and feed and water consumption analyses were performed.

Results

Acrylamide altered the morphology and histology of the small intestinal wall, decreasing proliferation, myenteron and submucosal thicknesses, villus length, fractal dimension, crypt depth, crypt number, and the small intestinal absorptive surface. Conversely, apoptosis, hemoglobin adduct levels, intensity of epithelium staining, enterocyte number, villus epithelial thickness, and crypt width and parameters associated with nerve ganglia were increased. The two potato fiber preparations that were used abolished the negative influences of acrylamide on the small intestinal wall and had no influence on the hemoglobin adduct levels of acrylamide.

Conclusion

The negative impact of acrylamide on the histologic structure, regeneration, and innervation of the small intestinal wall and the absorptive function of the small intestinal mucosa can be abolished by dietary potato fiber preparations.