- 作者：Xiaoxiao Zhou ; Ph.D.&lowast ; ; &Dagger ; ; Chao Zhang ; Ph.D.&dagger ; ; Xiaoqing Wang ; Ph.D.&Dagger ; ; Bingchen An ; Ph.D.&Dagger ; ; Peng Zhang ; Ph.D.§ ; ; Zhen’ ; an Zhu ; M.D.&Dagger ; ; zhuzhenan2008@126.com
- 关键词：polyethylene particles ; berberine ; lipopolysaccharide ; osteolysis ; calvarium
- 刊名：Journal of Surgical Research
- 出版年：2012
- 期刊代码：299_00224804
- 类别：med
- 出版时间：April, 2012
- 卷：173
- 期：2
- 页码：e47-e52
- 文件大小：210 K
lass="h4">Background
Wear particle-induced osteolysis could lead to the aseptic loosening of implants. Studies have suggested that endotoxins, such as lipopolysaccharides (LPS), may be the primary causes of wear particle-mediated osteolysis, and that osteolysis may originate from subclinical levels of bacterial infection. However, effective therapies against wear particles and gram-negative bacterial or LPS-induced bone resorption are limited.lass="h4">Materials and Methods
In the current study, the effect of berberine on LPS- and polyethylene (PE) particle-induced osteolysis in聽vivo was investigated using a mouse calvarial model. Osteoclast number per bone perimeter and eroded surface per bone surface were measured.
lass="h4">Results
Berberine (10 mg/kg), injected either simultaneously with LPS or 3 d after LPS (25 mg/kg) treatment, blocked LPS-induced osteoclast recruitment and bone resorption in the mouse calvarial model. A daily single-dose of berberine (10 mg/kg), injected either simultaneously with PE particles or 4 d after treatment with PE particles, blocked PE particle-induced osteoclast recruitment and bone resorption. Berberine treatment markedly decreased LPS and PE particle-induced osteoclast recruitment and bone resorption in the murine calvarial model.
lass="h4">Conclusion
These results suggest that berberine may have therapeutic effect for osteolysis induced by聽wear particles and LPS in gram-negative bacteria.