MiR-483-5p suppresses the proliferation of glioma cells via directly targeting ERK1

详细信息	查看全文 | 推荐本文 |

• 作者：Li Wang^a ; Ming Shi^a ; Shuangxing Hou^a ; Bojun Ding^a ; Lijuan Liu^a ; Xituan Ji^b ; Jian Zhang^c ; ^{biozhangj@yahoo.com.cn} ; Yanchun Deng^a ; ^{yanchundeng@yahoo.com}
• 关键词：miRNA ; microRNA ; ERK1 ; extracellular regulated kinase1 ; UTR ; untranslated region
• 刊名：FEBS Letters
• 出版年：2012
• 期刊代码：70_00145793
• 类别：bio
• 出版时间：7 May, 2012
• 卷：586
• 期：9
• 页码：1312-1317
• 文件大小：790 K

摘要

MicroRNAs (miRNAs) exhibit tumor-specific expression signatures and play crucial roles in tumorigenesis by targeting oncogenes. Here, through analyzing the miRNA-array profiles of human glioblastoma tissues and the adjacent normal brain tissues, we found miR-483-5p was significantly down-regulated in gliomas, which was confirmed in both human glioma specimens and cell lines. The overexpression of miR-483-5p suppressed glioma cell proliferation and induced a G0/G1 arrest. In contrast, miR-483-5p inhibition promoted cell proliferation. Furthermore, by a dual-luciferase reporter assay and expression analysis, we identified extracellular signal-regulated kinase 1 (ERK1) as a direct target of miR-483-5p. ERK1 knockdown can block cell proliferation induced by miR-483-5p inhibition. Thus, our findings provide the first evidence that miR-483-5p can serve as a tumor suppressor in gliomas.