Expression of serum amyloid A transcripts in human trophoblast and fetal-derived trophoblast-like choriocarcinoma cells
- 作者：Alenka Kovacevic ; Astrid Hammer ; Monika Sundl ; Bettina Pfister ; Andelko Hrzenjak ; Alpana Ray ; Bimal K. Ray ; Wolfgang Sattler and Ernst Malle
- 关键词：Acute-phase SAA ; Apolipoprotein ; Cytokine ; Inflammation ; Fetal development
- 刊名：FEBS Letters
- 出版年：2006
- 期刊代码：70_00145793
- 类别：bio
- 出版时间：9 January 2006
- 卷：580
- 期：1
- 页码：161-167
- 文件大小：1224 K
摘要
The placenta comprises a highly specialized trophoblast layer, which arises from the embryo and differentiates during embryonic development to perform specialized functions, e.g., synthesis of pregnancy-associated hormones, growth factors and cytokines. As there is no evidence of maternal acute-phase protein transplacental transfer and trophoblast plays an important role in regulating immune responses at the feto-maternal interface, the expression of acute-phase serum amyloid A (A-SAA) was investigated in human first trimester trophoblast and trophoblast-like JAR and Jeg-3 choriocarcinoma cells. We here show expression of cytokine receptors and cytokine-dependent induction of A-SAA in JAR and Jeg-3 cells. While interleukin-1α/β is a major agonist for A-SAA expression in JAR, tumor necrosis factor-α is the predominant agonist in Jeg-3. First trimester trophoblast and JAR/Jeg-3 cells further express the human homolog of SAA-activating factor-1, a transcription factor involved in cytokine-mediated induction of A-SAA genes. A-SAA1 and A-SAA2 transcripts were increased in first trimester trophoblast during pregnancy weeks 10 and 12 suggesting that A-SAA plays a role during early fetal development.