Endogenous CO₂ may inhibit bacterial growth and induce virulence gene expression in enteropathogenic Escherichia coli

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• 作者：Haydee Martí ; nez^a ; Thomas Buhse^b ; Marco Rivera^a ; P. Parmananda^c ; Guadalupe Ayala^d ; Joaquí ; n Sá ; nchez^e ; ^{joaquin.sanchez@microbio.gu.se}
• 关键词：Bacterial growth kinetics ; Bacterial growth auto-inhibition ; Carbon dioxide inhibited growth ; Culture surface-to-volume ratio ; Volatile bacterial growth inhibitor ; Carbon dioxide-induced virulence
• 刊名：Microbial Pathogenesis
• 出版年：2012
• 期刊代码：172_08824010
• 类别：bio
• 出版时间：July, 2012
• 卷：53
• 期：1
• 页码：49-55
• 文件大小：712 K

摘要

Analysis of the growth kinetics of enteropathogenic Escherichia coli (EPEC) revealed that growth was directly proportional to the ratio between the exposed surface area and the liquid culture volume (SA/V). It was hypothesized that this bacterial behavior was caused by the accumulation of an endogenous volatile growth inhibitor metabolite whose escape from the medium directly depended on the SA/V. The results of this work support the theory that an inhibitor is produced and indicate that it is CO₂. We also report that concomitant to the accumulation of CO₂, there is secretion of the virulence-related EspB and EspC proteins from EPEC. We therefore postulate that endogenous CO₂ may have an effect on both bacterial growth and virulence.