Evidence for a blockwise distribution of acetyl groups onto homogalacturonans from a commercial sugar beet (Beta vulgaris) pectin
- 作者：Marie-Christine Ralet ; Marie-Jeanne Cré ; peau ; Estelle Bonnin
- 关键词：Pectin ; Endo-polygalacturonase ; Oligogalacturonate ; Oligosaccharide ; Enzymatic fingerprinting ; Mass spectrometry
- 刊名：Phytochemistry
- 出版年：2008
- 期刊代码：85_00319422
- 类别：ch
- 出版时间：June 2008
- 卷：69
- 期：9
- 页码：1903-1909
- 文件大小：273 K
摘要
Commercial acid-extracted sugar beet pectin was extensively hydrolysed using an endo-polygalacturonase (AnPGI from Aspergillus niger or AnPGII from A. niger or FmPG from Fusarium moniliforme) in combination with Aspergillus aculeatus pectin methyl-esterase (AaPME). The homogalacturonan-derived oligogalacturonates released were quantified by high-performance anion-exchange chromatography and their structure determined by mass spectrometry. The different endo-polygalacturonases exhibited variable tolerance towards acetyl groups. AnPGI was the most active and FmPG the less. A hypothetical homogalacturonan was constructed using the AnPGI-recovered oligogalacturonates as building blocks and the validity of the model was checked taking into account FmPG observed requirements and hydrolysis products. A blockwise repartition of the acetyl groups onto sugar beet pectin homogalacturonan is proposed.