Synthetic resin-bound truncated Candida antarctica lipase B for production of fatty acid alkyl esters by transesterification of corn and soybean oils with ethanol or butanol

详细信息	查看全文 | 推荐本文 |

• 作者：Stephen R. Hughes^a ; ^{Stephen.Hughes@ars.usda.gov} ; Bryan R. Moser^b ; Samantha Robinson^c ; ^d ; Elby J. Cox^a ; Amanda J. Harmsen^c ; ^d ; Jon A. Friesen^c ; Kenneth M. Bischoff^a ; Marjorie A. Jones^c ; Rebecca Pinkelman^e ; Sookie S. Bang^e ; Ken Tasaki^d ; Kenneth M. Doll^b ; Nasib Qureshi^f ; Siqing Liu^a ; Badal C. Saha^f ; John S. Jackson^a ; Michael A. Cotta^f ; Joseph O. Rich^a ; Paolo Caimi^g
• 关键词：CALB ; Candida antarctica lipase B ; ORF ; open reading frame ; SBO ; soybean oil ; CO ; corn oil ; EE ; ethyl ester
• 刊名：Journal of Biotechnology
• 出版年：2012
• 期刊代码：53_01681656
• 类别：imm
• 出版时间：31 May, 2012
• 卷：159
• 期：1-2
• 页码：69-77
• 文件大小：969 K

摘要

A gene encoding a synthetic truncated Candida antarctica lipase B (CALB) was generated via automated PCR and expressed in Saccharomyces cerevisiae. Western blot analysis detected five truncated CALB variants, suggesting multiple translation starts from the six in-frame ATG codons. The longest open reading frame, which corresponds to amino acids 35-317 of the mature lipase, appeared to be expressed in the greatest amount. The truncated CALB was immobilized on Sepabeads^庐 EC-EP resin and used to produce ethyl and butyl esters from crude corn oil and refined soybean oil. The yield of ethyl esters was 4-fold greater from corn oil than from soybean oil and was 36%and 50%higher, respectively, when compared to a commercially available lipase resin (Novozym 435) using the same substrates. A 5:1 (v/v) ratio of ethanol to corn oil produced 3.7-fold and 8.4-fold greater yields than ratios of 15:1 and 30:1, respectively. With corn oil, butyl ester production was 56%higher than ethyl ester production. Addition of an ionic catalytic resin step prior to the CALB resin increased yields of ethyl esters from corn oil by 53%compared to CALB resin followed by ionic resin. The results suggest resin-bound truncated CALB has potential application in biodiesel production using biocatalysts.