Anti-inflammatory effects of polyunsaturated fatty acids in THP-1 cells

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• 作者：Guixiang Zhao ; Terry D. Etherton ; Keith R. Martin ; John P. Vanden Heuvel ; Peter J. Gillies ; Sheila G. West and Penny M. Kris-Etherton
• 关键词：n-6 and n-3 fatty acids ; Linoleic acid ; α ; -Linolenic acid ; Docosahexaenoic acid ; Inflammation ; Interleukin-6 ; Interleukin-1β ; Tumor necrosis factor-α ; NF-κ ; B ; PPARγ
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2005
• 期刊代码：159_0006291x
• 类别：bio
• 出版时间：2005
• 卷：336
• 期：3
• 页码：909-917
• 文件大小：320 K

摘要

The effects of linoleic acid (LA), α-linolenic acid (ALA), and docosahexaenoic acid (DHA) were compared to that of palmitic acid (PA), on inflammatory responses in human monocytic THP-1 cells. When cells were pre-incubated with fatty acids for 2-h and then stimulated with lipopolysaccharide for 24-h in the presence of fatty acids, secretion of interleukin (IL)-6, IL-1β, and tumor necrosis factor-α (TNFα) was significantly decreased after treatment with LA, ALA, and DHA versus PA (P < 0.01 for all); ALA and DHA elicited more favorable effects. These effects were comparable to those for 15-deoxy-Δ^12,14-prostaglandin J2 (15d-PGJ2) and were dose-dependent. In addition, LA, ALA, and DHA decreased IL-6, IL-1β, and TNFα gene expression (P < 0.05 for all) and nuclear factor (NF)-κB DNA-binding activity, whereas peroxisome proliferator-activated receptor-γ (PPARγ) DNA-binding activity was increased. The results indicate that the anti-inflammatory effects of polyunsaturated fatty acids may be, in part, due to the inhibition of NF-κB activation via activation of PPARγ.