Direct double antibody sandwich immunoassay of mucin M1 epitopes in human mucus secreting pancreatic cell lines
- 作者：Montserrat ; Colette ; Hollande ; Etienne ; Guy-Crotte ; Odette ; Figarella ; Catherine
- 关键词：Mucin M1 epitopes ; ELISA ; Biotin/streptavidin-peroxidase ; Cystic fibrosis
- 刊名：Clinica Chimica Acta
- 出版年：1995
- 期刊代码：54_00098981
- 类别：med
- 出版时间：December 15, 1995
- 卷：243
- 期：1
- 页码：43-52
- 文件大小：506 K
摘要
A direct sandwich enzyme immunoassay using two monoclonal antibodies was developed in order to quantify mucin M1 antigens produced by two pancreatic adenocarcinoma cell lines: CAPAN-1 and CFPAC-1. As a solid phase, the wells of a microtiter plate were coated with a first monoclonal antibody, 1-13 M1 and the biotinylated monoclonal conjugate 9-13 M1 was used as the second antibody. The assay was optimized with streptavidin-peroxidase. The detection limit of the assay is 1.6 ng/ml. This ELISA is highly specific, sensitive, reproducible and quickly performed. It will permit the comparison of mucin exocytosis by the two cell lines in response to secretagogue agents and may help in the study of the pathogenesis of mucus hypersecretion such as cystic fibrosis.